microfluidic tools for single cell analysis
A natural match in size-scale offers unique opportunities for using microfluidics to sample and analyze single cells and to study the role of single cell heterogeneity in various biological contexts. We are interested in developing single cell multi-omic tools using microfluidic approaches to enhance sensitivity, specificity and throughput of assays to make them single-cell measurement compatible.
PUBLICATIONS:
J. Rudge, N. Rafat, M. Hoyle, A. Sarkar, Electronics Immunoassays using Enzymatically Amplified Metallization on Microparticles, 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021), Palm Springs, USA, October 2021
Sarkar A, Wu L, Han J. Microfluidic Probes for Single Cell Proteomic Analysis. In: Open-Space Microfluidics: Concepts, Implementations, Applications. 1st ed. Wiley-VCH Verlag GmbH & Co. KGaA ; 2018. pp. 221-248. Publisher's Version
Wu L, Claas AM, Sarkar A, Lauffenburger DA, Han J. High-throughput protease activity cytometry reveals dose-dependent heterogeneity in PMA-mediated ADAM17. Integrative Biology [Internet]. 2015;7 :513-524
Cheow LF, Sarkar A, Kolitz S, Lauffenburger DA, Han J. Detecting Kinase Activities from Single Cell Lysate Using Conentration-Enhanced Mobility Shift Assay. Analytical Chemistry [Internet]. 2014;86 (15) :7455-7462.
Sarkar A*, Kolitz S*, Lauffenburger DA, Han J. Microfluidic Probe for Single Cell Analysis in Adherent Tissue Culture. Nature Communications [Internet]. 2014;5 :3421.