Publications & Conference Presentations
2024
Microscale Electronic Measurement of Functional Cellular Immunity for Point-Of-Care Diagnostics
Asma Hashim, Yuvraj Rallapalli, Yasir Alnakhli, Mallika Senthil, Aniruddh Sarkar
BMES Conference, Baltimore, MD, 2024.
IMPEDANCE BASED DETECTION OF INDIVIDUAL METALLIZED CELLS FOR POC DIAGNOSTICS
Yuvraj Rallapalli, Josiah Rudge, Asma Hashim, Madeline Hoyle, Mallika Senthil, Aniruddh Sarkar
BMES Conference, Baltimore, MD, 2024.
microparticle enabled single cell electronic immunophenotyping and selective electroporation
Madeline Hoyle, Josiah Rudge, Yuvraj Rallapalli, Aniruddh Sarkar
MicroTAS Conference, Montréal, Canada, 2024.
Anushka Saha, Trirupa Chakraborty, Javad Rahimikollu, Hanxi Xiao, Lorena B. Pereira de Oliveira, Timothy W. Hand, Sukwan Handali, W. Evan Secor, Lucia A. O. Fraga, Jessica K. Fairley, Jishnu Das, Aniruddh Sarkar
Science Translational Medicine, 2024.
2023
Multiplexed High Throughout Point-of-Care Biosensing of Onchocerciasis Antibody Markers
Mallika Senthil, Sarah Ali, Balazs Kaszala, Dhruti Trivedi, Neda Rafat, Sukwan Handali, Sylvia A. Ossai, Evan W. Secor, Aniruddh Sarkar
ASTMH Conference, Chicago, IL, 2023.Mycobacterium Leprae Antigen-Specific Antibody Profiling and Cytokine Analyses Reveal Unique Signatures of Leprosy As Well As Immune Changes with Schistosome Mansoni Co-Infections
Pedro Marcal, Anushka Saha, Trirupa Chakraborty, Lorena Oliveira, Lucia Fraga, Jishnu Das, Aniruddh Sarkar, Jessica Fairley
ASTMH Conference, Chicago, IL, 2023.
Comprehensive Antibody Profiling in Schistosomiasis Reveals Immunological Signatures of Active Infection
Anushka Saha, Trirupa Chakraborty, Sukwan Handali, William E. Secor, Lucia Alves de Oliveira Fraga, Jessica Fairley, Jishnu Das, Aniruddh Sarkar
ASTMH Conference, Chicago, IL, 2023.
Multiplexed Antigen Specific Antibody Fc Profiling for Point of Care Diagnosis of Tuberculosis
Sarah Ali, Preetham Peddireddy, Abhipsa Panigrahi, Asma Hashim, Aniruddh Sarkar
ASTMH Conference, Chicago, IL, 2023.
Electronic Immunoassay Using Enzymatic Metallization on Microparticles
J. Rudge, M. Hoyle, N. Rafat, A. Spitale, M. Honan, A. Sarkar
ACS Omega, 2023.
Comprehensive Antibody Profiling for Biomarker Discovery and Point-of-Care Diagnostics for NTDs
A. Saha, T. Chakraborty, S. Ali, M. Senthil, B. Kaszala, J. Fairley, L. Oliveira, L. Fraga, J. Das, A. Sarkar
Tropical Infectious Diseases Gordon Research Conference, Galveston, TX, 2023
Inexpensive High-Throughput Multiplexed Biomarker Detection Using Enzymatic Metallization with Cellphone-Based Computer Vision
N. Rafat, L. Brewer, N. Das, D. Trivedi, B. Kaszala, A. Sarkar
ACS Sensors, 2023.
2022
COMPREHENSIVE ANTIBODY PROFILING TO DEFINE IMMUNOLOGICAL SIGNATURES OF DISEASE STATE AND OUTCOME
S. P. Peddireddy, A. Saha, S. A. Rahman, T. Chabraborty, J. Das, A. Sarkar
Immunoengineering Gordon Research Conference, Ventura CA, 2022
COMPREHENSIVE ANTIBODY PROFILING PREDICTS REJECTION IN KIDNEY TRANSPLANT RECIPIENTS
A. Saha, T. Chabraborty, J. Das, A. Sarkar
2022 Annual Meeting of the Biomedical Engineering Society (BMES 2022), San Antonio TX, 2022
MULTIPLEXED HIGH THROUGHPUT POINT-OF-CARE BIOSENSING OF ONCHOCERCIASIS ANTIBODY BIOMARKERS
D. Trivedi, B. Kaszala, N. Rafat, S. Handali, S. Ossai, W.E. Secor, A. Sarkar
2022 Annual Meeting of the Biomedical Engineering Society (BMES 2022), San Antonio TX, 2022
IN-FLOW SINGLE CELL IMPEDANCE SPECTROSCOPY AND ELECTROPORATION USING A MICROSCALE APERTURE
J. Rudge, A. Sarkar
2022 Annual Meeting of the Biomedical Engineering Society (BMES2022), San Antonio, 2022
HIGH THROUGHPUT ELECTRONIC DETECTION OF COVID-19 BIOMARKERS USING ENZYMATIC METALLIZATION
H. Zhang, N. Rafat, J. Rudge, Y. Kim, A. Sarkar
2022 Annual Meeting of the Biomedical Engineering Society (BMES2022), San Antonio, 2022
A MULTIPLEXED HIGH-THROUGHPUT METHOD FOR POINT-OF-CARE BIOSENSING OF COVID-19 VACCINE EFFICACY
N. Rafat, D. Trivedi, B. Kaszala, L. Brewer, H. Zhang, A. Sarkar
2022 Annual Meeting of the Biomedical Engineering Society (BMES2022), San Antonio, 2022
N. Rafat, H. Zhang, J. Rudge, Y. Kim, S. P. Peddireddy, N. Das, A. Sarkar
Small, 2022
S. P. Peddireddy, S. A. Rehman, A. R. Cilio, G. M. Vijay, A. Somasundram, C. J. Workman, W. Bain, B. J. McVeryry, B. Methe, J. S. Lee, P. Ray, A. Ray, T. C. Bruno, D. A. A. Vignali, G. D. Kitsios, A. Morris, H. Singh, A. Sarkar, J. Das
Cell Reports, 39, 11020, 2022
2021
MULTIPLEXED POINT-OF-CARE ELECTRICAL DETECTION OF COVID-19 BIOMARKERS USING ENZYMATICALLY AMPLIFIED METALLIZATION ON NANOSTRUCTURED SURFACES
N. Rafat, H. Zhang, J. Rudge, Y. Kim, A. Sarkar
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021), Palm Springs, USA, October 2021
ELECTRONICS IMMUNOASSAYS USING ENZYMATICALLY AMPLIFIED METALLIZATION ON MICROPARTICLES
J. Rudge, N. Rafat, M. Hoyle, A. Sarkar
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021), Palm Springs, USA, October 2021
MULTIPLEXED ANTIBODY PROFILING PREDICTS OUTCOME IN SEVERE COVID-19
S. P. Peddireddy, S. Rehman, H. Singh, J. Das, A. Sarkar
2021 Annual Meeting of the Biomedical Engineering Society (BMES2020). Orlando, USA, October 2021
ELECTRONIC ENZYME LINKED IMMUNOSORBANT ASSAY FOR DETECTION OF COVID-19 BIOMARKERS
N. Rafat, H. Zhang, S. P. Peddireddy, A. Sarkar
American Chemical Society Fall Meeting (ACS Fall 2021), Atlanta, USA, August 2021.
2020
A FEEDBACK-CONTROLLED HIGH EFFICIENCY SINGLE CELL ELECTROPORATION CHIP FOR GENE AND CELL THERAPY
M. Hoyle, A. Sarkar
2020 Annual Meeting of the Biomedical Engineering Society (BMES2020). Virtual, 2020.
Sarkar A, Hou HW, Han J, Alter G.; 2019. System and method for multiplexed affinity purification of proteins and cells. United States of America patent 10228313B2. Publisher's Version
Jennewein MF, Goldfarb I, Dolatshahi S, Cosgrove C, Noelette FJ, Krykbaeva M, Das J, Sarkar A, Gorman MJ, Fischinger S, et al. Fc Glycan-Mediated Regulation of Placental Antibody Transfer. Cell. 2019;178 (1) :202-215.e14.
Sarkar A, Lu L, Han J, Alter G. Electrical Detection of Glycan Biomarkers for Point-of-Care Diagnosis of Tuberculosis. 2018 Annual Meeting of the Biomedical Engineering Society (BMES2018). 2018.
Sarkar A, Wu L, Han J. Microfluidic Probes for Single Cell Proteomic Analysis. In: Open-Space Microfluidics: Concepts, Implementations, Applications. 1st ed. Wiley-VCH Verlag GmbH & Co. KGaA ; 2018. pp. 221-248. Publisher's Version
Sarkar A, Lu L, Han J, Alter G. An Integrated Electrical Enzyme-linked Immunosorbent Assay (E2LISA) for Point-of-Care Diagnosis of Tuberculosis Using Glycan Biomarkers. 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2017). 2017.
Sarkar A, Hou HW, Han J, Alter G.; 2016. System and method for multiplexed affinity purification of proteins and cells. WIPO (World Intellectual Prop Org) patent 2016077055 A1. Full Text
Sarkar A, Lofano G, Blackburn S, Han J, Alter G. A Field-Portable, Inertio-Magnetic Microfluidic Cell Sorter for Rapid Discovery of Zika-Specific Antibodies, in 2016 Annual Meeting of the Biomedical Engineering Society (BMES2016). Minneapolis, USA ; 2016.
Sarkar A, Hou HW, Mahan AE, Han J, Alter G. Multiplexed affinity-based separation of proteins and cells using inertial microfluidics. Scientific Reports [Internet]. 2016;6 :23589. Full TextAbstract
Sarkar A, Hou HW, Han J, Alter G. Integrated Inertial and Magnetic Microfluidic Portable Cell Sorter for Infectious Disease Applications in Resource-Poor Settings, in 2015 Annual Meeting of the Biomedical Engineering Society (BMES2015). Tampa, USA ; 2015.
Wu L, Claas AM, Sarkar A, Lauffenburger DA, Han J. High-throughput protease activity cytometry reveals dose-dependent heterogeneity in PMA-mediated ADAM17. Integrative Biology [Internet]. 2015;7 :513-524. Full TextAbstract
Sarkar A, Hou HW, Mahan AE, Han J, Alter G. Inertial Microfluidics for Multiplexed Affinity Separation of Proteins and Cells, in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2014). San Antonio, USA ; 2014. Full Text
Cheow LF, Sarkar A, Kolitz S, Lauffenburger DA, Han J. Detecting Kinase Activities from Single Cell Lysate Using Conentration-Enhanced Mobility Shift Assay. Analytical Chemistry [Internet]. 2014;86 (15) :7455-7462. Full TextAbstract
Sarkar A*, Kolitz S*, Lauffenburger DA, Han J. Microfluidic Probe for Single Cell Analysis in Adherent Tissue Culture. Nature Communications [Internet]. 2014;5 :3421. Full TextAbstract
Chen C-H, Miller MA, Sarkar A, Beste MT, Isaacson KB, Lauffenburger DA, Griffith LG, Han J. Multiplexed Protease Activity Assay for Low Volume Clinical Samples Using Droplet Based Microfluidics and Its Application to Endometriosis. J Am Chem Soc [Internet]. 2013;135 (5) :1645-48. Full TextAbstract
Li LD, Crouzier T, Sarkar A, Dunphy L, Han J, Ribbeck K. Spatial Configuration and Composition of Charge Modulates Transport into a Mucin Hydrogel Barrier. Biophys J [Internet]. 2013;105 (6) :1357-65. Full TextAbstract
Sarkar A, Kolitz S, Lauffenburger DA, Han J. An Integrated Microfluidic Probe for Multiplexed Single Cell Kinase Activity Measurement, in International Conference on the Systems Biology of Human Disease (SBHD2012). Heidelberg, Germany ; 2012.
Chen C-H, Miller MA, Sarkar A, Beste MT, Lauffenburger DA, Griffith LG, Han J. High-Throughput Multiplexed Protease Activity Measurement using a Droplet Based Microfluidic Platform with Picoinjector. 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2012) [Internet]. 2012. Full Text
Sarkar A, Kolitz S, Lauffenburger, Douglas A., Han J. An Integrated Microfluidic Probe for Multiplexed Single Cell Kinase Activity Measurement. 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2012) [Internet]. 2012 :416-418. Full TextAbstract
Chen C, Sarkar A, Song Y, Miller MA, Kim SJ, Griffith LG, Lauffenburger DA, Han J. Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator, in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2011). Seattle, USA ; 2011. Full Text
Sarkar A, Kolitz S, Cheow LF, Lauffenburger DA, Han J. An Integrated Microfluidic Probe for Concentration-Enhanced Selective Single Cell Kinase Activity Measurement. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2011) [Internet]. 2011 :1394-1396. Full TextAbstract
Cheow LF, Sarkar A, Kolitz S, Lauffenburger D, Han J. Concentration-enhanced Mobility Shift Assay with Applications to Aptamer-based Biomarker Detection and Kinase Profiling. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2011) [Internet]. 2011. Full TextAbstract
Chen C-H, Sarkar A, Song Y-A, Miller MA, Kim SJ, Griffith LG, Lauffenburger DA, Han J. Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator. J Am Chem Soc. 2011;133 (27) :10368-71.Abstract
Sarkar A, Han J. Non-linear and Linear Enhancement of Enzymatic Reaction Kinetics Using a Nanofluidic Biomolecule Concentrator. Lab on a Chip [Internet]. 2011;11 (15) :2569-76. Full TextAbstract
Sarkar A, Kolitz S, Lauffenburger DA, Han J. Microfluidic Concentration-Enhanced Single Cellular Kinase Assay, in International Conference on the Systems Biology of Human Disease (SBHD2010). Boston, USA ; 2010.
Song Y-A, Sarkar A, Han J. Encapsulation of Biomolecules in Micro-droplets at Programmable Concentrations Using a Electrokinetic Concentrator. 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2008) [Internet]. 2008. Full TextAbstract
Sarkar A, Shastry A, Mitra B, Mulherkar R, Lal R. High efficiency electroporation in a microfabricated sense-porate aperture, in 25th Annual Convention of the Indian Association of Cancer Research (IACR 2006). Navi Mumbai, India ; 2006.
Sarkar A, Shastry A, Mitra B, Mulherkar R, Lal R. High Efficiency Electroporation in a Microfabricated Sense-Porate Aperture, in 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2006). Tokyo, Japan ; 2006.
Mali P*, Sarkar A*, Lal R. Facile Fabrication of Microfluidic Systems Using Electron Beam Lithography. Lab on a Chip [Internet]. 2006;6 (2) :310-315. Full TextAbstract
Mali* P, Sarkar* A, Lal R. Facile fabrication of microfluidic systems using electron beam lithography, in 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2005). Boston, USA ; 2005.
Sarkar A, Mitra B, Shastry A, Wadia S, Mulherkar R, Lal R. A Low Voltage Single Cell Electroporator with a Microfabricated Sense-Porate Aperture. 17th IEEE Conference on Micro Electro Mechanical Systems, Maastricht (MEMS 2004). 2004 :375–378.Abstract
Sarkar A, Mitra B, Shastry A, Wadia S, Mulherkar R, Lal R. A single cell electroporation system, in 12th International Workshop on the Physics of Semiconductor Devices (IWPSD-2003). Chennai, India ; 2003.