Electronic detection and manipulation techniques for various biological objects (e.g. biomolecules, cells)
Electronic sensing and actuation modalities offer unique advantages in biological and clinical applications including miniaturizability, integration and lower cost - riding on decades of advances made by the semiconductor and electronics industries. We are interested in developing microfabricated electronic biosensing and biomanipulation tools for a variety of biological objects ranging all the way in size scale from ions to biomolecules such as DNA, RNA and proteins to cells.
Applications include: Point-of-care (POC) electronic diagnostics for infectious diseases such as COVID-19, Tuberculosis and Neglected Tropical Diseases (NTDs e.g. schistosomiasis) especially for resource-poor settings. Portable, home-use and wearable diagnostic and monitoring technology in contexts ranging from soil, food and water quality to nutrition, infection, transplant rejection and cancer. Electronic delivery of macromolecules to cells for gene and cell therapy.
PUBLICATIONS:
N. Rafat, H. Zhang, J. Rudge, Y. Kim, S. P. Peddireddy, N. Das, A. Sarkar, Enhanced Enzymatically Amplified Metallization on Nanostructured Surfaces for Multiplexed Point-of-Care Electrical Detection of COVID-19 Biomarkers, Small, 2022
J. Rudge, A. Sarkar, In-Flow Single Cell Impedance Spectroscopy And Electroporation Using a Microscale Aperture, 2022 Annual Meeting of the Biomedical Engineering Society (BMES2022), San Antonio, 2022
H. Zhang, N. Rafat, J. Rudge, Y. Kim, A. Sarkar, High Throughput Electronic Detection of COVID-19 Biomarkers using Enzymatic Metallization, 2022 Annual Meeting of the Biomedical Engineering Society (BMES2022), San Antonio, 2022
J. Rudge, N. Rafat, M. Hoyle, A. Sarkar, Electronics Immunoassays using Enzymatically Amplified Metallization on Microparticles, 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021), Palm Springs, USA, October 2021
Sarkar A, Lu L, Han J, Alter G. Electrical Detection of Glycan Biomarkers for Point-of-Care Diagnosis of Tuberculosis. 2018 Annual Meeting of the Biomedical Engineering Society (BMES2018). 2018.